Reagents and Solutions

EcoArray solutions are designed to work with our hybridization protocol for cDNA nylon membrane arrays, regardless of species. The wash solutions are available in both 500 ml and 1 liter size bottles. Hybridization solution is available only in 100 ml bottles.

Wash #1. Low stringency wash solution. Contains 2X sodium saline citrate (SSC) and 0.5% sodium dodecyl sulfate (SDS). Approximately 150 ml is required for each membrane processed.

Wash #2. High stringency wash solution. Contains 0.5X sodium saline citrate (SSC) and 0.5% sodium dodecyl sulfate (SDS). Approximately 125 ml is required for each membrane processed.

"MemArray" Hybridization Buffer. EcoArray's MemArray Hybridization solution is specifically formulated for processing our cDNA nylon membrane arrays. This hybridization solution replaces Ambion's "UltraArray Hybridization Buffer" (#8666) which has been discontinued. Approximately 15 ml required for each membrane processed. It is available only in 100 ml size.