EcoArray has developed microarrays in several aquatic species to investigate environmental toxicology. The genes relevant to each species have been annotated and carefully curated. During our research and development of these arrays, we discovered that multiple probes designed for a specific gene do not respond equally (See Larkin et al, 2007). Therefore, EcoArray decided to spot only the best probe per gene, which serves the dual purposes of providing broad genome coverage while keeping the cost low. The genes are part of a wide variety of biological pathways and processes including: reproduction, cell communication, metabolism, homeostasis, and others.
These probes for each gene are spotted onto microarrays created for EcoArray by Agilent Technologies, a leading supplier of genomics and proteomics products. Using their SurePrint inkjet deposition of in situ synthesized 60-mer oligonucleotides, these arrays are manufactured on superior quality 1” x 3” (24.2 mm x 76.2 mm) treated glass slides for overall excellent performance. This process minimizes variability across each production lot (batch to batch) and provides the highest consistency in slide-to-slide comparisons. All of the glass slide microarrays are designed for either a two-color (cyanine-3 and cyanine-5) or one-color (cyanine-3) labeling reaction, the most reliable and sensitive methods for detecting differentially expressed genes.
For detailed information about each microarray, click on the blue text. For an alphabetical list of genes represented on each array, click on the appropriate blue button. Note that some of the pdf files are ~2 MB in size.
These microarrays are sold in two different package configurations:
• The Complete Package: With the complete package, you receive not only the arrays, but also complete sample processing in our laboratory. The package includes preparation and quality checks of the RNA samples, labeling with cyanine dyes, hybridization, scanning, quantitative estimates of gene expression with Agilent’s Feature Extraction software, and basic analyses with GeneSpring software. You will receive an EcoArray CD with the scanned images and metrics, raw data, analyzed data (graph, etc.), instructions on use of the databases, and customer support. Please contact EcoArray for a quote.
EcoArray will run a minimum of one slide for you, regardless of format.
• The Array Only Package: This package is intended for researchers who are very familiar with RNA extraction, target labeling and who have access to the specialized equipment (hybridization oven and chambers, and a high resolution 5 micron platform scanner) necessary for processing arrays. Please contact EcoArray for authorization to purchase the arrays directly from Agilent. The minimum number of arrays that must be purchased and run is determined by Agilent.
Labeling, Hybridization, and Washing: The recommended protocols for these arrays is, of course, that recommended by Agilent. The best results are achieved using their optimized reagents and kits. For a complete table or required and recommended protocols and equipment, click here.
Please contact us by email or get a quote online for academic or commercial pricing.
Register for the Data Sharing Program, if desired.
Data Sharing Program (DSP): The DSP gives you discounts on our products as well as a valuable analysis created by running your data against our database. You get top quality microarrays and we get access to your raw data files. You get valuable analysis and can publish under the MIAME standard (http://www.mged.org/Workgroups/MIAME/miame.html). For more details, click here.
